Abstract:

GC-MS was used to determine the composition and content of the terpene volatiles collected from uninfested and infested cones of Chinese pine (Pinus tabulaeformis) by means of solid phase microextraction (SPME) during the host-selection and ovipositive period of Dioryctria pryeri . The electroantennogram (EAG) of D. pryeri male and female adults was tested in responses to the uninfested and infested cone,and 6 terpene volatiles and their blends. The results showed that D. pryeri had a greater EAG response potential to the infested cone than to the uninfested cone,and EAG response of the mated female was greater than that of unmated female and male. Both female and male of D. pryeri had the greatest EAG response to blends of 1R-(+)-α-pinene+ β-myrcene+ D-(+)-limonene among the all tested agents, and to β-myrcene and D-(+)-limonene among the individual agents terpene components.In the same concentration of the terpene volatile, the male had a greater EAG response than unmated and mate female.

Key words: Dioryctria pryeri, cone of Pinus tabulaeformis, terpene volatiles, electroantennogram responses